Oxytocin Antibody

Product Discontinued

Oxytocin Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 11-60 of Human Neurophysin I.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OXT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Neurophysin I (K40) pAb in extracts from K562 cells.
  Immunohistochemistry (IHC) analyzes of Neurophysin I (K40) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Oxytocin Antibody

• MGC126890
• MGC126892
• Neurophysin I
• OT
• OT-NPI
• OXT
• Oxytocin
• oxytocin, prepro- (neurophysin I)
• oxytocin, prepropeptide
• oxytocin-neurophysin 1
• oxytocin-neurophysin I, preproprotein

Background

The nonapeptide hormones Arginine Vasopressin (ARG-Vasopressin or AVP) and Oxytocin are synthesized in the supraoptic and paraventricular nuclei of the hypothalamus together with their respective "carrier" proteins, the neurophysins. Vasopressin and Oxytocin are produced by separate populations of magnocellular neurons in both nuclei. Neurophysin I (NPI) and Neurophysin II (NPII) function as carrier proteins for Oxytocin and Vasopressin, respectively. Oxytocin is a pituitary hormone which induces uterine contractions during childbirth and the ejection of milk from the mammary glands during nursing. Vasopressin is involved in the metabolism of water and electrolytes and has been identified as a vasoconstrictor. Both Neurophysin genes exist as three exons, with each exon encoding a functional protein domain. Studies show that the identically conserved middle region (exon B) is involved in NP-NP homodimer formation as well as being the site for the Glycine 17 to Valine point mutation responsible for familial diabetes insipidus. The genes encoding Neurophysin I and II map to human chromosome 20p13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oxytocin Antibody (NBP1-19753)(1)

Publication using NBP1-19753

• Gujar AD, Ibrahim BA, Tamrakar P, Briski KP. Hypoglycemia differentially regulates hypothalamic glucoregulatory neurotransmitter gene and protein expression: Role of caudal dorsomedial hindbrain catecholaminergic input Neuropeptides 2013-03-09 [PMID: 23490004] (WB, Rat)

FAQs for Oxytocin Antibody (NBP1-19753). (Showing 1 - 1 of 1 FAQ).

1. I have a technical question regarding your oxytocin primary antibody (NBP1-19753). On the website you state that the immunogen is a synthetic peptide corresponding to residues surrounding Lysine 40 of the protein. I'm interested in detecting only the oxytocin peptide (residues 20-28). Am I correct in assuming that this antibody will not recognize this peptide? Do you have a primary antibody that will be specific for the oxytocin peptide rather than the oxytocin-neurophysin complex?
   • You are correct, NBP1-19753 will not recognize the oxytocin peptide, but GP-037-50 or NB600-667 should work just fine for you.

Bioinformatics

• Gene Symbol: OXT
• Entrez:
  • 5020(Human)
  • 24221(Rat)
• Uniprot:
  • P01178(Human)