Recombinant Human Otubain-1 Protein

Product Discontinued

Recombinant Human Otubain-1 Protein Summary

• Description: Recombinant protein for Human Otubain-1
  
  

  Source:E. coli
  Amino Acid Sequence:(N/A)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: OTUB1
• Purity: SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: Ki = 2. 5 nM vs UCH isopeptidase -T
• Theoretical MW: 8.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: An aqueous solution containing 0.15 M HCl
• Purity: SDS-PAGE

Alternate Names for Recombinant Human Otubain-1 Protein

• Deubiquitinating enzyme OTUB1
• EC 3.1.2
• EC 3.4.19.12
• FLJ20113
• FLJ40710
• hOTU1
• MGC111158
• OTB1
• OTB1MGC4584
• OTU domain, ubiquitin aldehyde binding 1
• OTU domain-containing ubiquitin aldehyde-binding protein 1
• OTU1
• OTU1OTU-domain Ubal-binding 1
• OTUB1
• Otubain1
• Otubain-1
• ubiquitin thioesterase OTUB1
• ubiquitin-specific protease otubain 1
• Ubiquitin-specific-processing protease OTUB1

Background

The C-terminal glycine carboxyl of Ubiquitin is synthetically modified to an aldehyde. Ubiquitin Aldehyde (Ub-H) is useful in the stabilization of ubiquitin-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts. Inhibition of deubiquitinylating enzyme activity by Ub-H can be used to identify and confirm such activity and to determine the inhibition kinetics for a particular enzyme. Recommended concentration for maximal inhibition is 2-5uM. Co-crystallization of ubiquitin aldehyde with specific deubiquitinylating enzymes (the inhibitor mimics the natural ubiquitin substrate) has also been used to probe enzyme:substrate interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUB1