OSMR beta Antibody (469229) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA
• Clone: 469229
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OSMR beta Antibody (469229) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human OSM R beta
  Glu28-Ser739
  Accession # Q99650
• Specificity: Detects human OSM R beta in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OSMR beta Antibody (469229) [Unconjugated]

• IL-31 receptor subunit beta
• IL-31R subunit beta
• IL-31RB
• IL-31R-beta
• Interleukin-31 receptor subunit beta
• MGC150627
• MGC75127
• oncostatin M receptor
• oncostatin-M specific receptor beta subunit
• oncostatin-M-specific receptor subunit beta
• OSM R beta
• OSMR beta
• OSMR
• OSMRB
• OSMRBMGC150626
• PLCA1

Background

OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta , respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha , G-CSF R, IL-6 R, IL-11 R alpha , and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta , gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).

1. Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379. 
2. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. 
3. Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39. 
4. Gearing, D.P. et al. (1992) Science 255:1434. 
5. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752. 
6. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.  
7. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635. 
8. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
9. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
10. Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
11. Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
12. Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
13. Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
14. Tanaka, M. et al. (2003) Blood 102:3154.
15. Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q99650()