OSBPL2 Antibody

Western Blot: OSBPL2 Antibody [NB100-1332] - NB100-1332 staining (1.5 ug/ml) of human heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

OSBPL2 Antibody Summary

• Description: Novus Biologicals Goat OSBPL2 Antibody (NB100-1332) is a polyclonal antibody validated for use in WB and ELISA. Anti-OSBPL2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence YFERNFSDCPDIY corresponding to C-Terminus according to NP_055650.1, NP_653081.1.
• Localization: Golgi Apparatus
• Specificity: This antibody is expected to recognise both reported isoforms (represented by NP_055650.1 and NP_653081.1)
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OSBPL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 2 ug/ml
• Application Notes: WB: Approx. 55-60 kDa band observed in human heart lysates (predicted MW of 54 and 55 kDa band according to NP_055650.1 and NP_653081.1).
• Control:

  OSBPL2 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OSBPL2 Antibody

• KIAA0772FLJ20223
• ORP-2MGC4307
• ORP2MGC8342
• OSBP-related protein 2
• oxysterol binding protein-like 2
• oxysterol-binding protein-like 2
• oxysterol-binding protein-related protein 2

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This encoded protein contains only the sterol-binding domain. In vitro studies have shown that the encoded protein can bind strongly to phosphatic acid and weakly to phosphatidylinositol 3-phosphate, but cannot bind to 25-hydroxycholesterol. The protein associates with the Golgi apparatus. Transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OSBPL2 Antibody (NB100-1332)(2)

Publications using NB100-1332

• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012-04-15 [PMID: 22241858] (WB, Mouse)
• Lehto M, Laitinen S, Chinetti G et al. The OSBP-related protein family in humans. J Lipid Res 2001-08-01 [PMID: 11483621]

Bioinformatics

• Gene Symbol: OSBPL2
• Entrez:
  • 9885(Human)
  • 228983(Mouse)
• Uniprot:
  • NP_055650(Human)