OS9 Antibody (JG38-07)

Western Blot: OS9 Antibody (JG38-07) [NBP2-75602] - Analysis of OS9 on mouse testis and rat testis tissue lysates using anti-OS9 antibody at 1/2,000 dilution.

Immunohistochemistry-Paraffin: OS9 Antibody (JG38-07) [NBP2-75602] - Analysis of paraffin-embedded rat epididymis tissue using anti-OS9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: OS9 Antibody (JG38-07) [NBP2-75602] - Analysis of paraffin-embedded human breast cancer tissue using anti-OS9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: OS9 Antibody (JG38-07) [NBP2-75602] - Analysis of paraffin-embedded human placenta tissue using anti-OS9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: OS9 Antibody (JG38-07) [NBP2-75602] - Analysis of paraffin-embedded mouse testis tissue using anti-OS9 antibody. Counter stained with hematoxylin.

Product Discontinued

OS9 Antibody (JG38-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human OS9 aa 561-667 / 667. (SwissProt: Q13438 Human; SwissProt: Q8K2C7 Mouse; SwissProt: Q5RKH6 Rat)
• Localization: Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: OS9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for OS9 Antibody (JG38-07)

• Amplified in osteosarcoma 9
• endoplasmic reticulum lectin 2
• ERLEC2
• erlectin 2
• OS-9
• osteosarcoma amplified 9, endoplasmic reticulum associated protein
• osteosarcoma amplified 9, endoplasmic reticulum lectin
• protein OS-9

Background

OS9 is a lectin which functions in endoplasmic reticulum quality control and ER-associated degradation. This ER glycoprotein is found in the intralumenal level and highly expressed in tumor tissue. The OS9 gene is transcriptionally induced upon activation of the Ire1/Xbp1 ER-stress pathway.

Functionally, OS9 binds to HIF-1, a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. It may also bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. One such possible target includes TRPV4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OS9