Recombinant Human Ornithine Carbamoyltransferase Protein

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SDS-Page: Ornithine Carbamoyltransferase Recombinant Protein [NBP1-99051]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Ornithine Carbamoyltransferase Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 33-354 of Human Ornithine Carbamoyltransferase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMNKVQL KGRDLLTLKN FTGEEIKYML WLSADLKFRI KQKGEYLPLL QGKSLGMIFE KRSTRTRLST ETGFALLGGH PCFLTTQDIH LGVNESLTDT ARVLSSMADA VLARVYKQSD LDTLAKEASI PIINGLSDLY HPIQILADYL TLQEHYSSLK GLTLSWIGDG NNILHSIMMS AAKFGMHLQA ATPKGYEPDA SVTKLAEQYA KENGTKLLLT NDPLEAAHGG NVLITDTWIS MGQEEEKKKR LQAFQGYQVT MKTAKVAASD WTFLHCLPRK PEEVDDEVFY SPRSLVFPEA ENRKWTIMAV MVSLLTDYSP QLQKPKF

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
OTC
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
20mM MES buffer (pH 6.0) containing 2mM DTT, 10% glycerol, 100mM NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Ornithine Carbamoyltransferase Protein

  • EC 2.1.3
  • EC 2.1.3.3
  • MGC129967
  • MGC129968
  • MGC138856
  • OCTD
  • ornithine carbamoyltransferase
  • ornithine carbamoyltransferase, mitochondrial
  • Ornithine transcarbamylase
  • OTCase

Background

OTC, also called ornithine carbamoyltransferase, belongs to the ATCase/OTCase family. OTC plays a vital role in the urea cycle, catalyzing the second step in this pathway: the formation of L-citrulline from L-orthinine and carbamoyl phosphate. In humans, the urea cycle is an important pathway to detoxification of ammonia. Mutations in the gene encoding OTC are associated with the X-linked disorder OTCD (ornithine carbamoyltransferase deficiency). OTCD is a disorder of the urea cycle characterized by hyperammonemia. Recombinant human OTC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol OTC