ORC3L Antibody

Western Blot: ORC3L Antibody [NBP2-19632] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ORC3L Antibody [NBP2-19632] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORC3L protein stained by ORC3L antibody [N1N3] diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: ORC3L Antibody [NBP2-19632] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:100 dilution.

Product Discontinued

ORC3L Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ORC3L. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (82%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ORC3L Antibody

• IMAGE50150
• LAT
• LATHEOorigin recognition complex, subunit 3-like (yeast)
• ORC3Lhomolog of latheo, Drosophila
• origin recognition complex subunit 3
• Origin recognition complex subunit Latheo
• origin recognition complex, subunit 3 (yeast homolog)-like
• origin recognition complex, subunit 3 honolog (yeast)
• origin recognition complex, subunit 3 honolog
• origin recognition complex, subunit 3
• origin recognition complex, subunit 3-like

Background

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ORC3
• Uniprot:
  • Q9UBD5(Human)