Oncomodulin Antibody

Western blot shows lysates of rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Rat Oncomodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Oncomodulin at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Oncomodulin Antibody Summary

• Immunogen: E. coli-derived recombinant rat Oncomodulin
  Met1-Ser109
  Accession # P02631
• Specificity: Detects rat Oncomodulin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Oncomodulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OCM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oncomodulin Antibody

• OCM
• OCM1
• OCMN
• OM
• ONCM
• Oncomodulin
• Parvalbumin beta

Background

Oncomodulin (OM; also parvalbumin beta) is a 12 - 14 kDa member of the parvalbumin family of Ca++binding proteins. It is expressed in early embryonic cells, placenta, and in tumors. OM was originally thought to have expression restricted to neoplastic tissues, early embryonic cells and certain tumor cell lines. Recent research shows that oncomodulin is also expressed and secreted by macrophages where, in the retina, it binds to retinal ganglion cells (RGCs) and functions to promote axon regenerationin early embryonic cells, placenta, and in tumors. OM is both cytoplasmic, and secreted. Rat OM is 109 amino acids (aa) in length. It contains a vestigial Ca++binding site (aa 733) and two EF hand domains, the latter of which contains one highaffinity Ca++binding site (aa 78108). Relative to parvalbumin alpha, OM has a lower pI (<4.8), a higher affinity for Ca++, and they share only 50% aa identity. Full length rat OM shares 95% and 89% aa identity with mouse and human OM, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OCM
• Uniprot:
  • P02631()