Western Blot: OLFM4 Antibody [NBP2-24535] - Analysis of human OLFM4 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using OLFM4 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: OLFM4 Antibody [NBP2-24535] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse stomach using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated ...read more
Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Analysis of OLFM4 in formalin-fixed, paraffin-embedded human liver tissue using OLFM4 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse liver using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated ...read more
Simple Western: OLFM4 Antibody [NBP2-24535] - Simple Western lane view shows a specific band for OLMF4 in 0.2 mg/ml of Human Liver (left) and Mouse Liver (right) lysate. This experiment was performed under reducing ...read more
Product Details
Summary
Reactivity
Hu, Mu, PmSpecies Glossary
Applications
WB, Simple Western, ICC/IF, IHC, IHC-P, Single-Cell Western
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
![Western Blot: OLFM4 Antibody [NBP2-24535] - Analysis of human OLFM4 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using OLFM4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize2/OLFM4-Antibody-Western-Blot-NBP2-24535-img0001.jpg "Western Blot: OLFM4 Antibody [NBP2-24535] - Analysis of human OLFM4 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using OLFM4 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: OLFM4 Antibody [NBP2-24535] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-OLFM4 (NBP2-24535) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/OLFM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24535-img0004.jpg "Immunocytochemistry/Immunofluorescence: OLFM4 Antibody [NBP2-24535] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-OLFM4 (NBP2-24535) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse stomach using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated very specific staining for OLFM4 in almost all cell types. The signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm. The simple columnar epithelial cells showed stronger and a distinct inter-cellular signal compared to the cells of the gastric glands.](http://images.novusbio.com/fullsize2/OLFM4-Antibody-Immunohistochemistry-Paraffin-NBP2-24535-img0006.jpg "Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse stomach using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated very specific staining for OLFM4 in almost all cell types. The signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm. The simple columnar epithelial cells showed stronger and a distinct inter-cellular signal compared to the cells of the gastric glands.")
![Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Analysis of OLFM4 in formalin-fixed, paraffin-embedded human liver tissue using OLFM4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize2/OLFM4-Antibody-Immunohistochemistry-Paraffin-NBP2-24535-img0002.jpg "Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Analysis of OLFM4 in formalin-fixed, paraffin-embedded human liver tissue using OLFM4 antibody at 5 ug/ml.")
![Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse liver using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice staining in all the hepatocytes and the signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm.](http://images.novusbio.com/fullsize2/OLFM4-Antibody-Immunohistochemistry-Paraffin-NBP2-24535-img0005.jpg "Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse liver using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice staining in all the hepatocytes and the signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm.")
![Simple Western: OLFM4 Antibody [NBP2-24535] - Simple Western lane view shows a specific band for OLMF4 in 0.2 mg/ml of Human Liver (left) and Mouse Liver (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize2/OLFM4-Antibody-Simple-Western-NBP2-24535-img0003.jpg "Simple Western: OLFM4 Antibody [NBP2-24535] - Simple Western lane view shows a specific band for OLMF4 in 0.2 mg/ml of Human Liver (left) and Mouse Liver (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.")
![Immunohistochemistry-Paraffin: GCAP1 Antibody [NBP2-55158] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/GCAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55158-img0003.jpg)
![Immunohistochemistry-Paraffin: GCAP1 Antibody [NBP2-55158] - Staining of human duodenum shows no positivity in glandular cells as expected.](http://images.novusbio.com/images2/GCAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55158-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GUCY2D Antibody (1E6) [H00003000-M06] - Analysis of monoclonal antibody to GUCY2D on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/GUCY2D-Antibody-(1E6)-Immunocytochemistry-Immunofluorescence-H00003000-M06-img0003.jpg)
![ELISA: GUCY2D Antibody (1E6) [H00003000-M06] - Detection limit for recombinant GST tagged GUCY2D is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/images2/GUCY2D-Antibody-(1E6)-ELISA-H00003000-M06-img0001.jpg)
![ELISA: GUCY2D Antibody (1E6) [H00003000-M06] - Detection limit for recombinant GST tagged GUCY2D is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/images2/GUCY2D-Antibody-(1E6)-ELISA-H00003000-M06-img0002.jpg)
![Western Blot: GRIM19 Antibody [NBP1-90050] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/GRIM19 Antibody-Western Blot-NBP1-90050-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GRIM19 Antibody [NBP1-90050] - Staining of human cell line U-251 MG shows positivity in mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/GRIM19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90050-img0004.jpg)
![Immunohistochemistry-Paraffin: GRIM19 Antibody [NBP1-90050] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/images2/GRIM19-Antibody-NBP1-90050-img0009.jpg)
![Western Blot: GCAP2 Antibody [NBP2-68721] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/images2/GCAP2-Antibody-Western-Blot-NBP2-68721-img0001.jpg)
![Immunohistochemistry-Paraffin: GCAP2 Antibody [NBP2-68721] - Staining of human retina shows strong cytoplasmic positivity in cones and outer plexiform layer.](http://images.novusbio.com/images2/GCAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68721-img0002.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the Rhodopsin protein and appearance of more of this high molecular weight material.](http://images.novusbio.com/images2/Rhodopsin-Antibody-(B630)-Western-Blot-NBP2-25160-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).](http://images.novusbio.com/images2/Rhodopsin Antibody (B630)-Immunocytochemistry-Immunofluorescence-NBP2-25160-img0002.jpg)
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Western-Blot-NB100-56583-img0004.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0008.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/images2/CEACAM5-CD66e-Antibody-(CEA31)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0003.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/images2/CEACAM5-CD66e-Antibody-(CEA31)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0001.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/images2/CEACAM5-CD66e-Antibody-(CEA31)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0002.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")