Olfactomedin-1/Noelin-1 Antibody [Alexa Fluor® 594]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Olfactomedin-1/Noelin-1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Olfactomedin‑1/Noelin‑1
  Ser2-Arg481
  Accession # NP_062371
• Specificity: Detects mouse Olfactomedin‑1/Noelin‑1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Olfactomedin‑1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as Olfactomedin-1 or pancortin, is a 75 kDa secreted extracellular matrix glycoprotein expressed in the brain (1‑3). Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1‑4) that are combinations of a common central region with alternate N- and C-termini (3, 4). Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled-coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain (4). In the species tested, these isoforms appear to oppose actions of the longer isoforms (5). All isoforms are capable of forming homo‑ or heterodimers through cysteines in their common central region (2). The C‑terminus of Noelin-1 (mouse aa 482‑485) contains a putative endoplasmic reticulum retention sequence that has not been included in the R&D Systems protein (6). Mouse Noelin-1 (aa 17‑481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species (1). In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells (7). Overproduction of Noelin-1 prolongs neural crest cell formation (7). Noelin-1 is also expressed in mouse neural crest, but not frog (1‑3). All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia (1‑3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.