OCTN2/SLC22A5 Antibody (893753) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human OCTN2/SLC22A5 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human OCTN2/SLC22A5 Monoclonal Antibody (Catalog # MAB11004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 893753
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OCTN2/SLC22A5 Antibody (893753) [Unconjugated] Summary

• Immunogen: NS0 transfected with human SLC22A5
  Accession # O76082
• Specificity: Detects human OCTN2/SLC22A5 in ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OCTN2/SLC22A5 Antibody (893753) [Unconjugated]

• CDSP
• FLJ46769
• High-affinity sodium-dependent carnitine cotransporter
• OCTN2
• OCTN2high-affinity sodium dependent carnitine cotransporter
• OCTN2VT
• organic cation transporter 2
• organic cation transporter 5
• Organic cation/carnitine transporter 2
• SCD
• SLC22A5
• solute carrier family 22 (organic cation transporter), member 5
• solute carrier family 22 (organic cation/carnitine transporter), member 5
• solute carrier family 22 member 5

Background

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for the elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A5 is an integral plasma membrane protein which functions as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. Mutations in SLC22A5 are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O76082(Human)