![Western Blot: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using NBP2-24637. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Nup53-Antibody-Western-Blot-NBP2-24637-img0002.jpg "Western Blot: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using NBP2-24637. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
| Reactivity | Hu, Mu, Bv, Ca, Pm, Rt, Ch, ZeSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | Novus Biologicals Rabbit Nup53 Antibody (NBP2-24637) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A portion of amino acids 280-326 of human NUP35 was used as the immunogen. |
| Predicted Species | Rat (100%), Chicken (94%), Zebrafish (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NUP35 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP2-24637-img0001.jpg "Immunohistochemistry-Paraffin: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.")
![N/A Neutrophil Elastase/ELA2 [Biotin]](https://images.novusbio.com/images2/DATA_Neutrophil_Elastase_DY451705_ELISA_2461.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")