NUP50 Overexpression Lysate (Native)

Images

 
Western Blot: NUP50 Overexpression Lysate (Adult Normal) [NBP2-10010] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NUP50.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NUP50 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_705931. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens NUP50, mRNA.
Gene
NUP50

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NUP50

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NUP50 Overexpression Lysate (Native)

  • 50 kDa nucleoporin
  • MGC39961
  • NPAP60
  • NPAP60LNucleoporin Nup50
  • nuclear pore complex protein Nup50
  • Nuclear pore-associated protein 60 kDa-like
  • nuclear pore-associated protein 60L
  • nucleoporin 50kD
  • nucleoporin 50kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-83213
Species: Hu
Applications: WB, IHC, IHC-P
NB400-148
Species: Hu, Mu, Rt, Po, ChHa, Pm
Applications: WB, EM, ICC/IF, IHC, IHC-P
NB100-93329
Species: Hu
Applications: WB, ICC/IF, IP
H00001434-M08
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP2-00532
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-85383
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-93325
Species: Hu
Applications: WB, IP
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
AF1555
Species: Hu
Applications: WB
NBP1-88941
Species: Hu
Applications: IHC, IHC-P
NBP1-31776
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-84735
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81546
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-48546
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45570
Species: Hu
Applications: WB, Flow, IHC
H00003836-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
MAB8209
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-10010
Species: Hu
Applications: WB

Publications for NUP50 Lysate (NBP2-10010) (0)

There are no publications for NUP50 Lysate (NBP2-10010).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NUP50 Lysate (NBP2-10010) (0)

There are no reviews for NUP50 Lysate (NBP2-10010). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NUP50 Lysate (NBP2-10010) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NUP50 Products

Bioinformatics Tool for NUP50 Lysate (NBP2-10010)

Discover related pathways, diseases and genes to NUP50 Lysate (NBP2-10010). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NUP50 Lysate (NBP2-10010)

Discover more about diseases related to NUP50 Lysate (NBP2-10010).
 

Pathways for NUP50 Lysate (NBP2-10010)

View related products by pathway.

PTMs for NUP50 Lysate (NBP2-10010)

Learn more about PTMs related to NUP50 Lysate (NBP2-10010).

Blogs on NUP50

There are no specific blogs for NUP50, but you can read our latest blog posts.

Customers Who Bought This Also Bought

NUP50 Antibody
NB100-1212
POMC Antibody
NB100-1533

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NUP50 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol NUP50