NUP50 Antibody

Western Blot: NUP50 Antibody [H00010762-B01P] - Analysis of NUP50 expression in human liver.

Immunocytochemistry/Immunofluorescence: NUP50 Antibody [H00010762-B01P] - Analysis of purified antibody to NUP50 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: NUP50 Antibody [H00010762-B01P] - Analysis of purified antibody to NUP50 on formalin-fixed paraffin-embedded human pancreatic cancer. (antibody concentration 1.5 ug/ml)

Western Blot: NUP50 Antibody [H00010762-B01P] - Analysis of NUP50 expression in transfected 293T cell line by NUP50 polyclonal antibody. Lane 1: NUP50 transfected lysate(51.48 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

NUP50 Antibody Summary

• Immunogen: NUP50 (NP_009103.2, 1 a.a. - 468 a.a.) full-length human protein. MAKRNAEKELTDRNWDQEDEAEEVGTFSMASEEVLKNRAIKKAKRRNVGFESDTGGAFKGFKGLVVPSGGGRFSGFGSGAGGKPLEGLSNGNNITSAPPFASAKAAADPKVAFGSLAANGPTTLVDKVSNPKTNGDSQQPSSSGLASSKACVGNAYHKQLAALNCSVRDWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA
• Specificity: NUP50 - nucleoporin 50kDa,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: NUP50
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NUP50 Antibody

• 50 kDa nucleoporin
• MGC39961
• NPAP60
• NPAP60LNucleoporin Nup50
• nuclear pore complex protein Nup50
• Nuclear pore-associated protein 60 kDa-like
• nuclear pore-associated protein 60L
• nucleoporin 50kD
• nucleoporin 50kDa

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUP50
• Entrez:
  • 10762(Human)
• Uniprot:
  • NP_009103.2(Human)