Nucleoporin NUP85 Antibody

Western Blot: Nucleoporin NUP85 Antibody [NB100-1355] - (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Nucleoporin NUP85 Antibody Summary

• Description: Novus Biologicals Goat Nucleoporin NUP85 Antibody (NB100-1355) is a polyclonal antibody validated for use in WB and ELISA. Anti-Nucleoporin NUP85 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ARAIIREGSLEGS corresponding to C-Terminus according to NP_079120.1.
• Localization: Nuclear
• Specificity: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NUP85
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in HeLa lysates (calculated MW of 75 kDa band according to NP_079120).
• Control:

  HeLa Whole Cell Lysate
  Nucleoporin NUP85 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nucleoporin NUP85 Antibody

• 85 kDa nucleoporin
• FLJ12549
• FROUNT
• nuclear pore complex protein Nup85
• nucleoporin 85kDa
• Nucleoporin Nup75
• Nucleoporin Nup85
• Nucleoporin
• Nup75
• NUP85
• PCNT
• PCNT1
• Pericentrin-1

Background

Pericentrin is probably a conserved integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nucleoporin NUP85 Antibody (NB100-1355)(2)

Publications using NB100-1355

• Cao Y, Wu X, Wang X et al. Transmembrane dynamics of the Thr-5 phosphorylated sarcolipin pentameric channel. Archives of biochemistry and biophysics 2017-05-09 [PMID: 27378083]
• Doxsey SJ, Stein P, Evans L et al. Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Cell 1994-02-25 [PMID: 8124707]

Bioinformatics

• Gene Symbol: NUP85
• Entrez:
  • 79902(Human)
  • 18541(Mouse)
• Uniprot:
  • NP_079120.(Human)