NTH1 Antibody

Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human NTH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NTH1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NTH1 Antibody Summary

• Immunogen: E. coli-derived recombinant human NTH1
  Met1-Leu312
  Accession # P78549
• Specificity: Detects human NTH1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NTHL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NTH1 Antibody

• EC 4.2.99.18
• endonuclease III-like protein 1
• nth endonuclease III-like 1 (E. coli)
• NTH1
• NTHL1
• OCTS3
• OCTS3NTH1nth (E.coli endonuclease III)-like 1

Background

NTH1 (Endonuclease III) is a DNA glycosylase with AP lyase activity. It functions as an enzyme to repair oxidative damage to DNA through the BER (base excision repair) pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NTH1 Antibody (AF2675)(1)

Publication using AF2675

• Saha T, Rih JK, Roy R Transcriptional regulation of the base excision repair pathway by BRCA1. J. Biol. Chem., 2010-02-25;285(25):19092-105. 2010-02-25 [PMID: 20185827] (Western Blot, Human)

Bioinformatics

• Gene Symbol: NTHL1
• Uniprot:
  • P78549()