nSMase Antibody [Janelia Fluor® 635]

Product Discontinued

nSMase Antibody [Janelia Fluor® 635] Summary

• Immunogen: Produced in rabbits immunized with E. coli-derived Human nSMase fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMPD2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen and protein A Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for nSMase Antibody [Janelia Fluor® 635]

• EC 3.1.4.12
• ISC1
• lyso-PAF-PLC
• Lyso-platelet-activating factor-phospholipase C
• Neutral sphingomyelinase
• NSMASE
• N-SMase
• NSMASE1
• sphingomyelin phosphodiesterase 2
• sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)

Background

Sphingomyelin is also present in all eukaryotic cell membranes, especially the plasma membrane, and is particularly concentrated in the nervous system because sphingomyelin is a major component of myelin, the fatty insulation wrapped around nerve cells by Schwann cells or oligodendrocytes. Multiple Sclerosis is a disease characterised by deterioration of the myelin sheath, leading to impairment of nervous conduction. nSMase (Neutral sphingomyelinase; Sphingomyelin phosphodiesterase 2) converts sphingomyelin to ceramide. Although widely expressed in all tissues examined, except the spleen, high enzymatic activity occurs only in the brain. Mice lacking Smpd2 and Smpd3 are completely devoid of neutral SMase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMPD2