NSF Antibody (9G7-3) - BSA Free

Western Blot: NSF Antibody (9G7-3) [NBP1-19333] - Western blot analysis: Lane 1: MW marker, Lane 2: HeLa (Heat Shocked), Lane 3: Vero, Lane 4: CHO-K1, Lane 5: ESK-4.

Immunohistochemistry: NSF Antibody (9G7-3) [NBP1-19333] - Immunohistochemistry analysis of human testis tissue stained with NSF, mAb (9G7-3) at 20ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Rb, Sh
• Applications: WB, IHC
• Clone: 9G7-3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NSF Antibody (9G7-3) - BSA Free Summary

• Immunogen: Recombinant bovine NSF protein
• Specificity: NSF (9G7-3)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NSF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000

Reactivity Notes

Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Pig, Rabbit, Sheep. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: In PBS, pH 7.2, containing 50% glycerol.
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for NSF Antibody (9G7-3) - BSA Free

• EC 3.6.4.6
• NEM-sensitive fusion protein
• N-ethylmaleimide-sensitive factor
• N-ethylmaleimide-sensitive factor-like protein
• N-ethylmaleimide-sensitive fusion protein
• SKD2
• vesicle-fusing ATPase
• Vesicular-fusion protein NSF

Background

N ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NSF
• Entrez:
  • 4905(Human)
  • 18195(Mouse)
  • 60355(Rat)