| Reactivity | HuSpecies Glossary |
| Applications | IHC, IHC-P |
| Clone | MM0495-8E25 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | FITC |
| Immunogen | Human NrCAM |
| Marker | Neuronal Marker |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NRCAM |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Diseases for NrCAM Antibody (NBP2-11826F)Discover more about diseases related to NrCAM Antibody (NBP2-11826F).
| Pathways for NrCAM Antibody (NBP2-11826F)View related products by pathway.
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PTMs for NrCAM Antibody (NBP2-11826F)Learn more about PTMs related to NrCAM Antibody (NBP2-11826F).
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![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3 (same as 123C3.D5)) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)](http://images.novusbio.com/fullsize2/NCAM-1-CD56-Antibody-(123C3-(same-as-123C3.D5))-Immunohistochemistry-Paraffin-NBP2-15184-img0016.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3 (same as 123C3.D5)) [NBP2-15184] - Flow Cytometry: NCAM-1/CD56 Antibody (123C3 (same as 123C3.D5)) [Alexa Fluor® 647] [NBP2-33132AF647] - A surface stain was performed on SH-SY5Y cells with NCAM-1/CD56 Antibody (123C3) NBP2-33132AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes.Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize2/NCAM-1-CD56-Antibody-(123C3-(same-as-123C3.D5))-Flow-Cytometry-NBP2-15184-img0017.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3 (same as 123C3.D5)) [NBP2-15184] - analysis of CD56 on human monocytes using CD56 antibody at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize2/NCAM-1-CD56-Antibody-(123C3-(same-as-123C3.D5))-Flow-Cytometry-NBP2-15184-img0004.jpg)
![Western Blot: FAM49B Antibody [NBP1-88582] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize2/FAM49B-Antibody-Western-Blot-NBP1-88582-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAM49B Antibody [NBP1-88582] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize2/FAM49B Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88582-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM49B Antibody [NBP1-88582] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.](http://images.novusbio.com/fullsize2/FAM49B-Antibody-Immunohistochemistry-Paraffin-NBP1-88582-img0007.jpg)
![Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Fibronectin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-FR and IF (Alexa Fluor488) was performed using NBP1-91258 at 1:400 dilution. Image was captured by epifluorescent microscopy. Image from verified customer review.](http://images.novusbio.com/fullsize2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - ICC-IF analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.](http://images.novusbio.com/fullsize2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section : astocyte and human nuclei. Image from a confirmed customer review.](http://images.novusbio.com/fullsize2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ZNF160 Antibody [NBP1-83939] - Staining of human cell line A-431 shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/ZNF160-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83939-img0006.jpg)
![Immunohistochemistry-Paraffin: ZNF160 Antibody [NBP1-83939] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize2/ZNF160-Antibody-Immunohistochemistry-Paraffin-NBP1-83939-img0005.jpg)
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: An intracellular stain was performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize2/Tenascin-C-Antibody-(4C8MS)-Flow-(Intracellular)-NB110-68136-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Tenascin-C-Antibody-(4C8MS)-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize2/Tenascin-C-Antibody-(4C8MS)-Immunohistochemistry-Paraffin-NB110-68136-img0009.jpg)
![Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.](http://images.novusbio.com/fullsize2/Synaptophysin-Antibody-Western-Blot-NB300-653-img0001.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Mouse Brain- Synaptophysin.](http://images.novusbio.com/fullsize2/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0005.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.](http://images.novusbio.com/fullsize2/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0002.jpg)
![Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.](http://images.novusbio.com/fullsize2/Tau-Antibody-(2E9)-Western-Blot-NBP2-25162-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.](http://images.novusbio.com/fullsize2/Tau-Antibody-(2E9)-Immunocytochemistry-Immunofluorescence-NBP2-25162-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.](http://images.novusbio.com/fullsize2/Tau-Antibody-(2E9)-Immunocytochemistry-Immunofluorescence-NBP2-25162-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - ICC-IF analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.](http://images.novusbio.com/images2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0007.jpg "Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - ICC-IF analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.")