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NRBF2 Antibody (OTI1A2) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
OTI1A2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73054AF350
Alexa Fluor 405
NBP2-73054AF405
Alexa Fluor 488
NBP2-73054AF488
Alexa Fluor 532
NBP2-73054AF532
Alexa Fluor 594
NBP2-73054AF594
Alexa Fluor 647
NBP2-73054AF647
Alexa Fluor 700
NBP2-73054AF700
Alexa Fluor 750
NBP2-73054AF750
Biotin
NBP2-73054B
DyLight 350
NBP2-73054UV
DyLight 405
NBP2-73054V
DyLight 488
NBP2-73054G
DyLight 550
NBP2-73054R
DyLight 594
NBP2-73054DL594
DyLight 680
NBP2-73054FR
DyLight 755
NBP2-73054IR
HRP
NBP2-73054H
Unconjugated
NBP2-46258
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46258
Azide and BSA Free
NBP2-73054
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NRBF2 Antibody (OTI1A2) [DyLight 650] Summary

Immunogen
Full length recombinant protein of human NRBF2 (NP_110386) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NRBF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for NRBF2 Antibody (OTI1A2) [DyLight 650]

  • Comodulator of PPAR and RXR
  • COPR
  • COPR1
  • COPR2
  • DKFZp564C1664
  • FLJ30395
  • NRBF-2
  • nuclear receptor binding factor 2
  • nuclear receptor binding factor-2
  • nuclear receptor-binding factor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD55/DAF was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 15 µg/mL Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CD55/DAF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (left panel, positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (right panel, negative staining) using Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(5 Publications)
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Applications: ICC, IHC
Calretinin Antibody
NBP1-88220
Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-88220] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Western Blot: Calretinin Antibody [NBP1-88220] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in cells in granular layer.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
CD21 Antibody
(SC0681)
NBP2-67605
Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.
(1 Publication)
Species: Hu, Pm, Mu
Applications: ICC/IF, IHC, IHC-P, IP, ISH, WB
CRIPTO Antibody -
BSA Free
NB100-1598
Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin
Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
(3 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, WB
DHRS4 Antibody
NBP2-98675
Immunohistochemistry-Paraffin: DHRS4 Antibody [NBP2-98675] - Immunochemical staining of human DHRS4 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: DHRS4 Antibody [NBP2-98675] - Immunochemical staining of human DHRS4 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Western Blot: DHRS4 Antibody [NBP2-98675] - DHRS4 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate 4 ul anti-DHRS4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DHRS4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa
Species: Hu
Applications: ELISA, ICC/IF, IHC-P, IP, WB
PPAR alpha/NR1C1
Antibody (3B6/PPAR) - ...
NB300-537
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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(12 Publications)
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Applications: ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
COPR5 Antibody
NBP2-30884
Immunohistochemistry-Paraffin: COPR5 Antibody [NBP2-30884] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
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Immunohistochemistry-Paraffin: COPR5 Antibody [NBP2-30884] - Staining of human prostate shows moderate nuclear positivity in glandular cells.
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alpha COP I
Antibody
NBP1-89705
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Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705] - Staining of human pancreas shows strong positivity.
Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705] - Staining of human stomach shows moderate granular cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NCOR2 Antibody
NB120-5802
Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
Procalcitonin
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NB120-14817
Procalcitonin Antibody (44D9)
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H00010966-P01
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NB200-322
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p120-catenin
Antibody (OTI2E8)
NBP2-45646
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Immunohistochemistry: p120-catenin Antibody (2E8) [NBP2-45646] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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NRBP2 Antibody
(4D1)
H00340371-M01
Western Blot: NRBP2 Antibody (4D1) [H00340371-M01] - Analysis of NRBP2 over-expressed 293 cell line, cotransfected with NRBP2 Validated Chimera RNAi ( Cat # H00340371-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRBP2 monoclonal antibody (M01), clone 4D1 (Cat # H00340371-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: NRBP2 Antibody (4D1) [H00340371-M01] - Analysis of NRBP2 expression in transfected 293T cell line by NRBP2 monoclonal antibody (M01), clone 4D1.Lane 1: NRBP2 transfected lysate(29.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: NRBP2 Antibody (4D1) [H00340371-M01] - Detection limit for recombinant GST tagged NRBP2 is approximately 0.03ng/ml as a capture antibody.
Species: Hu
Applications: ELISA, KD, S-ELISA, WB
p130Cas/BCAR1
Antibody (OTI1A9)
NBP2-00532
Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody, and then analyzed by flow cytometry.
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB

CSE1L/CAS/Exportin-2 A
H00001434-M08
Western Blot: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Analysis of CSE1L expression in Hela S3 NE (Cat # L013V3).
Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Mouse prostate tissue section stained with primary antibody for 10 hrs. IHC-P image submitted by a verified customer review.
ELISA: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Detection limit for recombinant GST tagged CSE1L is approximately 3ng/ml as a capture antibody.
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NRBF2 Antibody
(OTI1A2) [DyLight 650
NBP2-73054C
NRBF2 Antibody (OTI1A2) [DyLight 650]
Species: Hu, Mu, Rt
Applications: WB

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NRBF2