Recombinant Human NRAS Protein

SDS-Page: Recombinant Human NRAS Protein [NBP1-41158]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human NRAS Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-186 of Human NRAS
  
  

  Source: E.coli

  Amino Acid Sequence: MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV REIRQYRMKK LNSSDDGTQG CMGLPC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NRAS
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 20.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 100 mM NaCl, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human NRAS Protein

• ALPS4
• GTPase NRas
• HRAS1
• neuroblastoma RAS viral (v-ras) oncogene homolog
• N-ras protein part 4
• N-ras
• NRAS1
• NS6
• Transforming protein N-Ras
• v-ras neuroblastoma RAS viral oncogene homolog

Background

Neuroblastoma RAS viral (v-ras) oncogene homolog, also known as NRAS, is a member of the RAS gene family. NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Recombinant human NRAS was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for NRAS Recombinant Protein (NBP1-41158)(1)

Publication using NBP1-41158

• Boldogh I, Hajas G, Aguilera-Aguirre L et al. Activation of ras signaling pathway by 8-oxoguanine DNA glycosylase bound to its excision product, 8-oxoguanine J Biol Chem 2012-06-15 [PMID: 22568941] (IA)

Bioinformatics

• Gene Symbol: NRAS