NRAS Antibody (2G9)

Images

 
Western Blot: NRAS Antibody (2G9) [NBP1-74044] - The extracts of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat ...read more
Immunocytochemistry/ Immunofluorescence: NRAS Antibody (2G9) [NBP1-74044] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
2G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

NRAS Antibody (2G9) Summary

Immunogen
Recombinant human NRAS (1-186aa) purified from E. coli
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NRAS
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Control
NRAS Knockout 293T Cell Lysate
NRAS Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for NRAS Antibody (2G9)

  • ALPS4
  • GTPase NRas
  • HRAS1
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • N-ras protein part 4
  • N-ras
  • NRAS1
  • NS6
  • Transforming protein N-Ras
  • v-ras neuroblastoma RAS viral oncogene homolog

Background

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NRAS Antibody (NBP1-74044) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-74044AF350
Alexa Fluor 405 NBP1-74044AF405
Alexa Fluor 488 NBP1-74044AF488
Alexa Fluor 532 NBP1-74044AF532
Alexa Fluor 594 NBP1-74044AF594
Alexa Fluor 647 NBP1-74044AF647
Alexa Fluor 700 NBP1-74044AF700
Alexa Fluor 750 NBP1-74044AF750
Allophycocyanin NBP1-74044APC
Allophycocyanin/Cy7 NBP1-74044APCCY7
Biotin NBP1-74044B
DyLight 350 NBP1-74044UV
DyLight 405 NBP1-74044V
DyLight 488 NBP1-74044G
DyLight 550 NBP1-74044R
DyLight 594 NBP1-74044DL594
DyLight 650 NBP1-74044C
DyLight 680 NBP1-74044FR
DyLight 755 NBP1-74044IR
FITC NBP1-74044F
HRP NBP1-74044H
Janelia Fluor 549 NBP1-74044JF549
Janelia Fluor 646 NBP1-74044JF646
PE NBP1-74044PE
PE/Atto594 NBP1-74044PEATT594
PE/Cy5.5 NBP1-74044PECY55
PE/Cy7 NBP1-74044PECY7
PerCP NBP1-74044PCP

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Bioinformatics Tool for NRAS Antibody (NBP1-74044)

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Bioinformatics

Gene Symbol NRAS
Uniprot
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