NRAS Antibody (2G9)

Images

 
Western Blot: NRAS Antibody (AT2G9) [NBP1-74044] The extracts of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat ...read more
Immunocytochemistry/ Immunofluorescence: NRAS Antibody (2G9) [NBP1-74044] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
2G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

NRAS Antibody (2G9) Summary

Immunogen
Recombinant human NRAS (1-186aa) purified from E. coli
Specificity
Anti-human RAS mAb, clone 2G9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NRAS protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NRAS
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for NRAS Antibody (2G9)

  • ALPS4
  • GTPase NRas
  • HRAS1
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • N-ras protein part 4
  • N-ras
  • NRAS1
  • NS6
  • Transforming protein N-Ras
  • v-ras neuroblastoma RAS viral oncogene homolog

Background

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NRAS
Uniprot