NRAS Antibody (2G9) - BSA Free

Western Blot: NRAS Antibody (2G9) [NBP1-74044] - The extracts of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS(AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.

Immunocytochemistry/Immunofluorescence: NRAS Antibody (2G9) [NBP1-74044] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Product Discontinued

NRAS Antibody (2G9) - BSA Free Summary

• Immunogen: Recombinant human NRAS (1-186aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRAS
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NRAS Antibody (2G9) - BSA Free

• ALPS4
• GTPase NRas
• HRAS1
• neuroblastoma RAS viral (v-ras) oncogene homolog
• N-ras protein part 4
• N-ras
• NRAS1
• NS6
• Transforming protein N-Ras
• v-ras neuroblastoma RAS viral oncogene homolog

Background

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRAS
• Uniprot:
  • NP_002515(Human)