NPY4R Knockout HeLa Cell Lysate

Product Discontinued

NPY4R Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,4 bp deletion in exon 2 and 1 bp deletion in exon 2 and
• Gene: NPY4R

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for NPY4R Knockout HeLa Cell Lysate

• neuropeptide Y receptor type 4
• NPY4R
• NPY4RMGC116897
• pancreatic polypeptide receptor 1NPY4-R
• PP1Y4
• PPYR1

Background

NPY4R belongs to the Neuropeptide Receptor GPCR Family and may regulate central nervous system, cardiovascular, and gastrointestinal function. NPY4R has been reported to be expressed in bladder, brain, colon, endometrium, heart, lung, nasal mucosa, penis, prostate, skeletal muscle, small intestine, stomach, and vessel. ESTs have been isolated from human colon and head/neck libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NPY4R