NPRA/NPR1 Antibody (377029) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 377029
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NPRA/NPR1 Antibody (377029) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NPRA/NPR1
  Gly33-Glu473
  Accession # P16066
• Specificity: Detects human NPRA/NPR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse NPR1 or recombinant human NPR2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NPRA/NPR1 Antibody (377029) [Unconjugated]

• ANPa
• ANP-A
• ANPRA
• ANPR-A
• ANPRAEC 4.6.1.2
• atrial natriuretic peptide receptor 1
• Atrial natriuretic peptide receptor type A
• atrionatriuretic peptide receptor A
• GC-A
• Guanylate cyclase A
• GUC2A
• GUCY2A
• natriuretic peptide A type receptor
• natriuretic peptide receptor A
• natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptidereceptor A)
• NPR1
• NPRA
• NPR-A
• Pndr

Background

Natriuretic peptide receptor A/guanylate cyclase A (NPR1), also called NPRA or GC-A, is a 120‑140 kDa type I transmembrane glycoprotein that is the primary receptor for natrietic peptides ANP and BNP. Binding of ANP to the extracellular ligand binding domain (aa 54‑415), plus ATP to the intracellular kinase homology domain (aa 528‑805) activates a cytoplasmic guanylate cyclase (aa 840‑1023). NPR1 is expressed most highly in kidney, adrenal and adipose tissue. Human NPR1 extracellular domain shows 86%, 44% and 34% aa identity with mouse NPR1, human NPRB and human NPRC, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPRA/NPR1 Antibody (MAB4860)(1)

Publication using MAB4860

• S Zhang, DT Hansen, JM Martin-Gar, JD Zook, S Pan, FM Craciunesc, JC Burnett, P Fromme Purification, characterization, and preliminary serial crystallography diffraction advances structure determination of full-length human particulate guanylyl cyclase A receptor Scientific Reports, 2022-07-12;12(1):11824. 2022-07-12 [PMID: 35821229] (Western Blot, Insect)

Bioinformatics

• Gene Symbol: NPR1
• Uniprot:
  • P16066()