NPAS2 Antibody

Images

 
Western Blot: NPAS2 Antibody [NBP1-31363] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: NPAS2 Antibody [NBP1-31363] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:500. Red: ...read more
Immunohistochemistry-Frozen: NPAS2 Antibody [NBP1-31363] - Frozen sectioned E13.5 Rat brain. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, ...read more
Western Blot: NPAS2 Antibody [NBP1-31363] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: NPAS2 Antibody [NBP1-31363] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.
Immunohistochemistry-Paraffin: NPAS2 Antibody [NBP1-31363] - Paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Frozen: NPAS2 Antibody [NBP1-31363] - Frozen sectioned adult mouse retina. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

NPAS2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human NPAS2. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NPAS2 Antibody

  • Basic-helix-loop-helix-PAS protein MOP4
  • BHLHE9
  • bHLHe9MGC71151
  • Class E basic helix-loop-helix protein 9
  • Member of PAS protein 4
  • member of PAS superfamily 4
  • MOP4PAS domain-containing protein 4
  • neuronal PAS domain protein 2
  • neuronal PAS domain-containing protein 2
  • Neuronal PAS2
  • PASD4FLJ23138

Background

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NPAS2