NOX5 Antibody

Western Blot: NOX5 Antibody [NBP1-68862] - NBP1-68862 (0.5ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: NOX5 Antibody [NBP1-68862] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing staining within the cytoplasm and plasma membrane . The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/Immunofluorescence: NOX5 Antibody [NBP1-68862] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing staining within the cytoplasm and plasma membrane . The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Bv, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NOX5 Antibody Summary

• Immunogen: Peptide with sequence C-EWHPFTISSAPEQKD corresponding to internal region according to NP_078781.3, NP_001171708.1, NP_001171709.1.
• Epitope: C-EWHPFTISSAPEQKD
• Specificity: This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NOX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human testis lysates (calculated MW of 86.4 kDa band according to NP_078781.3).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32165681).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NOX5 Antibody

• EC 1.6.3.-
• MGC149776
• MGC149777
• NADPH oxidase 5
• NADPH oxidase, EF-hand calcium binding domain 5
• NOX5
• NOX5A
• NOX5B

Background

NOX5 is a novel NADPH oxidase that generates superoxide and functions as a H+ channel in a Ca(2+)-dependentmanner.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOX5 Antibody (NBP1-68862)(3)

Publications using NBP1-68862

• Jha JC, Dai A, Garzarella J et al. Independent of Renox, NOX5 Promotes Renal Inflammation and Fibrosis in Diabetes by Activating ROS-sensitive Pathways Diabetes 2022-03-11 [PMID: 35275988]
• Kim Yr, Kim Js, Gu Sj et Al. Identification of highly potent and selective inhibitor, TIPTP, of the p22phox-Rubicon axis as a therapeutic agent for rheumatoid arthritis Sci Rep 2020-03-12 [PMID: 32165681] (WB, IF, Mouse)
• Choi H, Kim S, Kim HJ et al. Sphingosylphosphorylcholine down-regulates filaggrin gene transcription through NOX5-based NADPH oxidase and cyclooxygenase-2 in human keratinocytes. Biochem Pharmacol 2010-07-01 [PMID: 20230798]

Bioinformatics

• Gene Symbol: NOX5
• Entrez:
  • 79400(Human)
• Uniprot:
  • NP_078781.3(Human)