NOTUM Antibody - BSA Free

Western Blot: NOTUM Antibody [NBP1-77060] - Human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/mL.

Immunohistochemistry-Paraffin: NOTUM Antibody [NBP1-77060] - Human brain tissue with NOTUM antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: NOTUM Antibody - BSA Free [NBP1-77060] - Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NOTUM Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM. The immunogen is located within amino acids 90 - 140 of NOTUM. Amino Acid Squence: CNDGSPAGYYLKESRGSRR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOTUM
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1ug/ml
• Control Peptide:

  NOTUM Peptide (NBP1-77060PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for NOTUM Antibody - BSA Free

• EC 3.-
• notum pectinacetylesterase homolog (Drosophila)
• Notum
• protein notum homolog

Background

In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOTUM Antibody (NBP1-77060)(1)

Publication using NBP1-77060

• MJ Jeyarajah, G Jaju Bhatt, RD Kelly, KJ Baines, A Jaremek, FP Yang, H Okae, T Arima, V Dumeaux, SJ Renaud The multifaceted role of GCM1 during trophoblast differentiation in the human placenta Proceedings of the National Academy of Sciences of the United States of America, 2022-11-28;119(49):e2203071119. 2022-11-28 [PMID: 36442132]

Bioinformatics

• Gene Symbol: NOTUM
• Uniprot:
  • NP_848588(Human)