Nogo-B Antibody (523808) [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: IHC
• Clone: 523808
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

Nogo-B Antibody (523808) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Nogo-B.
  Met1-Ile183 (predicted)
  Accession # Q99P72
• Specificity: Detects mouse Nogo-B in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) Nogo-B and recombinant rat Nogo-A and no cross-reactivity with rhNogo-A is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nogo-B Antibody (523808) [Alexa Fluor® 647]

• ASY;Nbla00271;Nbla10545;NI220/250;NOGO;NSP;NSP-CL;Reticulon-4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X
• NogoB
• Nogo-B
• RTN4B

Background

Nogo-B, also known as ASY, is a 55 kDa splice form of Nogo-A/Reticulon-4 and is primarily expressed in vascular endothelial cells. Nogo-A contains a large N‑terminal cytoplasmic domain and a reticulon region that consists of two transmembrane segments with an intervening noncytoplasmic domain. Nogo-B lacks most of the N-terminal domain but retains the reticulon domain. It inhibits neointimal hyperplasia in the vasculature and is downregulated in atherosclerotic tissue and thoracic arterial aneurisms. Interaction of Nogo-B with NgBR promotes endothelial cell proliferation and migration. Within aa 1‑183, mouse Nogo-B shares 76% and 94% aa sequence identity with human and rat Nogo-B, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.