![Western Blot: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/Nogo Antibody (JM02-34)-Western Blot-NBP2-75595-img0009.jpg "Western Blot: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, IP |
| Clone | JM02-34 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant Protein |
| Localization | Endoplasmic reticulum membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RTN4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for Nogo Antibody (NBP2-75595)Discover more about diseases related to Nogo Antibody (NBP2-75595).
| Pathways for Nogo Antibody (NBP2-75595)View related products by pathway.
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PTMs for Nogo Antibody (NBP2-75595)Learn more about PTMs related to Nogo Antibody (NBP2-75595).
| Research Areas for Nogo Antibody (NBP2-75595)Find related products by research area.
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Nogo: A Promising Target for New Gene Therapies Nogo is a neurite outgrowth inhibitor protein that plays an important role during central nervous system (CNS) development as well as in endoplasmic reticulum signaling regulation. Studies using Nogo antibodies have revealed Nogo proteins regulate... Read full blog post. |
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| Gene Symbol | RTN4 |
![Immunocytochemistry/Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Immunocytochemistry-Immunofluorescence-NBP2-75595-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded mouse brain tissue using anti-Nogo antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Immunohistochemistry-Paraffin-NBP2-75595-img0008.jpg "Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded mouse brain tissue using anti-Nogo antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of Hela cells with Nogo antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Flow-Cytometry-NBP2-75595-img0001.jpg "Flow Cytometry: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of Hela cells with Nogo antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).")
![Immunocytochemistry/Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Immunocytochemistry-Immunofluorescence-NBP2-75595-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Immunocytochemistry-Immunofluorescence-NBP2-75595-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded human breast tissue using anti-Nogo antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Immunohistochemistry-Paraffin-NBP2-75595-img0005.jpg "Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded human breast tissue using anti-Nogo antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Nogo antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Immunohistochemistry-Paraffin-NBP2-75595-img0006.jpg "Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Nogo antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded mouse testis tissue using anti-Nogo antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Nogo-Antibody-(JM02-34)-Immunohistochemistry-Paraffin-NBP2-75595-img0007.jpg "Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded mouse testis tissue using anti-Nogo antibody. Counter stained with hematoxylin.")
![Western Blot: OMgp Antibody [NBP1-82483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/images2/OMgp-Antibody-Western-Blot-NBP1-82483-img0007.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0021.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/images2/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0008.jpg)
![Western Blot: MOG Antibody [H00004340-B01P] - Analysis of MOG expression in rat brain.](http://images.novusbio.com/images2/MOG-Antibody-Western-Blot-H00004340-B01P-img0003.jpg)
![Western Blot: MOG Antibody [H00004340-B01P] - Analysis of MOG expression in transfected 293T cell line by MOG polyclonal antibody. Lane 1: MOG transfected lysate(32.56 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/MOG Antibody-Western Blot-H00004340-B01P-img0004.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0015.jpg)
![Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/images2/Exosome-component-10-Antibody-Immunohistochemistry-Paraffin-NBP1-32870-img0010.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.](http://images.novusbio.com/images2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RTN3 Antibody [NBP1-88871] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/images2/RTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88871-img0005.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex, colon, liver and lymph node using Anti-RTN3 antibody (A) NBP1-88871 shows similar protein distribution across tissues to independent antibody NBP1-88870 (B).](http://images.novusbio.com/images2/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88871-img0018.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/images2/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88871-img0014.jpg)
![Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] - Breast cancer cells. Lane 1: MDA-MB231 + Negative control siRNA. Lane 2: MDA-MB231 + siELK3-1. Lane 3: MDA-MB231 + siELK3-2. Image from verified customer review.](http://images.novusbio.com/images2/ELK3-Antibody-(OTI1H3)-Western-Blot-NBP2-01264-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: ELK3 Antibody (1H3) [NBP2-01264] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.](http://images.novusbio.com/images/ELK3 Antibody (1H3)-Immunocytochemistry-Immunofluorescence-NBP2-01264-img0008.jpg)
![Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Human tonsil using anti-ELK3 mouse monoclonal antibody.](http://images.novusbio.com/images/ELK3 Antibody (1H3)-Immunohistochemistry-Paraffin-NBP2-01264-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: EBPL Antibody [NBP2-13943] - Immunofluorescent staining of human cell line PC-3 shows localization to endoplasmic reticulum.](http://images.novusbio.com/images2/EBPL Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13943-img0003.jpg)
![Immunohistochemistry-Paraffin: EBPL Antibody [NBP2-13943] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/images2/EBPL-Antibody-Immunohistochemistry-Paraffin-NBP2-13943-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RTN1 Antibody (MON162) [NBP1-97677] - Immunostaining of NCI-H82 variant small cell lung cancer](http://images.novusbio.com/images2/RTN1 Antibody (MON162)-Immunocytochemistry-Immunofluorescence-NBP1-97677-img0001.jpg)
![Immunohistochemistry-Frozen: RTN1 Antibody (MON162) [NBP1-97677] - Immunohistochemistry on frozen section of swine brain](http://images.novusbio.com/images2/RTN1-Antibody-(MON162)-Immunohistochemistry-Frozen-NBP1-97677-img0002.jpg)
![Western Blot: SLC10A3 Antibody [NBP1-84957] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-529](http://images.novusbio.com/images2/SLC10A3-Antibody-Western-Blot-NBP1-84957-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC10A3 Antibody [NBP1-84957] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/images2/SLC10A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84957-img0006.jpg)
![SDS-Page: Recombinant Human ATP5G3 Protein [H00000518-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images2/Recombinant-Human-ATP5G3-Protein-SDS-Page-H00000518-P01-img0001.jpg)
![Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.](http://images.novusbio.com/images2/RhoA-Antibody-Western-Blot-NB100-91273-img0034.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/images2/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0024.jpg)
![Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/images2/RhoA Antibody-Immunohistochemistry-NB100-91273-img0040.jpg)
![Western Blot: CDP/CUTL1 Antibody [NBP2-13883] - Analysis in human thyroid gland tissue.](http://images.novusbio.com/images2/CDP-CUTL1-Antibody-Western-Blot-NBP2-13883-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CDP/CUTL1 Antibody [NBP2-13883] - Staining of human cell line MCF7 shows localization to nucleoplasm and the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/images2/CDP-CUTL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13883-img0006.jpg)
![Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody [NBP2-13883] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/CDP-CUTL1-Antibody-Immunohistochemistry-Paraffin-NBP2-13883-img0011.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")