Nogo Antibody (JM02-34)

Images

 
Western Blot: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded mouse brain tissue using anti-Nogo antibody. Counter stained with hematoxylin.
Flow Cytometry: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of Hela cells with Nogo antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunocytochemistry/ Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: Nogo Antibody (JM02-34) [NBP2-75595] - Staining Nogo in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded human breast tissue using anti-Nogo antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Nogo antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Nogo Antibody (JM02-34) [NBP2-75595] - Analysis of paraffin-embedded mouse testis tissue using anti-Nogo antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
JM02-34
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Nogo Antibody (JM02-34) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant Protein
Localization
Endoplasmic reticulum membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RTN4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Nogo Antibody (JM02-34)

  • ASY
  • foocen
  • Human NogoA
  • KIAA0886
  • My043 protein
  • Nbla00271
  • Nbla10545
  • neurite growth inhibitor 220
  • Neurite outgrowth inhibitor
  • Neuroendocrine-specific protein C homolog
  • Neuroendocrine-specific protein
  • NI220/250
  • Nogo protein
  • NOGO-A
  • Nogo-B
  • NOGOC
  • Nogo-C
  • NOGORTN4-B1
  • NSP
  • NSP-CL
  • reticulon 4
  • reticulon 5
  • reticulon-4
  • reticulon-5
  • RTN4-A
  • RTN4-B2
  • RTN4-C
  • RTN-x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Diseases for Nogo Antibody (NBP2-75595)

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Research Areas for Nogo Antibody (NBP2-75595)

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Blogs on Nogo.

Nogo: A Promising Target for New Gene Therapies
Nogo is a neurite outgrowth inhibitor protein that plays an important role during central nervous system (CNS) development as well as in endoplasmic reticulum signaling regulation. Studies using Nogo antibodies have revealed Nogo proteins regulate...  Read full blog post.

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Bioinformatics

Gene Symbol RTN4