NMNAT-1 Antibody - BSA Free

Western Blot: NMNAT-1 Antibody [NBP2-32107] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-NMNAT1 antibody NBP2-32107 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32107] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NMNAT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NMNAT-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NMNAT-1 Antibody - BSA Free (NBP2-32107) is a polyclonal antibody validated for use in IHC and WB. Anti-NMNAT-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 229 to 279 of human Nicotinamide Nucleotide Adenylyltransferase 1 using the numbering given in entry NP_073624.2 (GeneID 64802).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NMNAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NMNAT-1 Antibody - BSA Free

• EC 2.7.7.1
• EC 2.7.7.18
• NaMN adenylyltransferase 1
• nicotinamide mononucleotide adenylyltransferase 1
• nicotinamide nucleotide adenylyltransferase 1
• nicotinamide nucleotide adenylyltransferase
• Nicotinate-nucleotide adenylyltransferase 1
• NMN adenylyltransferase 1
• NMNAT1
• NMNAT-1
• NMNATPNAT1
• PNAT1
• pyridine nucleotide adenylyltransferase 1

Background

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMNAT-1 Antibody (NBP2-32107)(1)

Publication using NBP2-32107

• Shi X, Jiang Y, Kitano A Et Al. Nuclear NAD+ homeostasis governed by NMNAT1 prevents apoptosis of acute myeloid leukemia stem cells Science advances 2021-07-01 [PMID: 34290089] (WB)

Bioinformatics

• Gene Symbol: NMNAT1
• Uniprot:
  • NP_073624.2(Human)