NMDAR2A Antibody (N327/95) [FL550] - Chimeric

• Reactivity: Hu, Mu, Rt, Rb
• Applications: ICC/IF, IHC
• Clone: N327/95
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: FL550
• Concentration: 0.5 mg/ml

NMDAR2A Antibody (N327/95) [FL550] - Chimeric Summary

• Description: FL550 Excitation: 550 nm, Emission: 575nm.
  Each order is a unique lot. For specialized requirements, please inquire for available options.
  
  This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N327/95 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N327/95 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NMDAR2A (Uniprot#: Q00959) produced recombinantly in E. Coli
• Specificity: Does not cross-react with GluN2B/NR2B
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: GRIN2A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for NMDAR2A Antibody (N327/95) [FL550] - Chimeric

• GluN2A
• glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• hNR2A
• NMDA receptor subtype 2A
• N-methyl D-aspartate receptor subtype 2A
• N-methyl-D-aspartate receptor subunit 2A
• subunit epsilon-1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN2A