NKCC1/SLC12A2 Antibody

Western Blot: NKCC1/SLC12A2 Antibody [NB100-75623] - E. coli-derived fusion protein as test antigen. Affinity-purified IgY dilution 1:2000, Goat anti-IgY-HRP dilution 1:1000. Colorimetric method for signal development (anti-SLC12A2).

Immunohistochemistry: NKCC1/SLC12A2 Antibody [NB100-75623] - Human colon tissue was incubated with anti-NKCC1 antibody at 5 ug/mL (1:200) overnight at 4C and detected with Alexa Fluor 488 Goat anti-Chicken (1:200 Invitrogen, cat# A11039). Nuclei stained with DAPI. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NKCC1/SLC12A2 Antibody Summary

• Immunogen: The immunogen for this antibody was a sequence from 800a.a. to 1000a.a. from NKCC1(a 1212a.a. long protein)
• Localization: Membrane; multi-pass membrane protein.
• Specificity: Chicken Polyclonal to NKCC1
• Clonality: Polyclonal
• Host: Chicken
• Gene: SLC12A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Immunohistochemistry
• Application Notes: Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution 1:2000. Goat anti-IgY-HRP dilution 1:1000. Colorimetric method for signal development.

Reactivity Notes

This antibody is reactive against Human, dog, rat, cow, mouse, chimpanzee.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NKCC1/SLC12A2 Antibody

• Basolateral Na-K-Cl symporter
• BSC
• BSC2
• Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
• NKCC1
• NKCC1MGC104233
• SLC12A2
• solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• solute carrier family 12 member 2

Background

NKCC1 (Na

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for NKCC1/SLC12A2 Antibody (NB100-75623) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Edward van Beelen ICC Human 09/24/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: human colon tissue,colon
• Species: Human
• Lot: 118K1873

Comments

• Comments: Human colon tissue was blocked with 5% BSA and incubated with anti-NKCC1 antibody at 5µg/ml (1:200 dilution [Novus Biologicals, cat# NB100-75623, lot# 118K1873]) overnight at 4°C and detected with Alexa Fluor Goat anti-Chicken 488 (1:200 dilution [Invitrogen, cat# A11039, lot# 745483]). Nuclei were counterstained with DAPI (blue). Cells were imaged using a 20x objective. As negative control, secondary antibody was omitted.

FAQs for NKCC1/SLC12A2 Antibody (NB100-75623). (Showing 1 - 1 of 1 FAQ).

1. Do you have any data with this antibody used on immunohistochemistry?
   • This particular antibody (NB100-75623) has so far only been tested in western blot. This does not mean that it will not work in IHC, just that we haven't tested it yet. Therefore, there is currently no data using NB100-75623 in IHC. If you would like to go ahead and test this antibody in IHC, note that you would be eligible for our Innovator's Reward program. Alternatively, here are our NKCC1 antibodies that have been validated in IHC.

Bioinformatics

• Gene Symbol: SLC12A2
• Entrez:
  • 6558(Human)
  • 20496(Mouse)
  • 83629(Rat)
• Uniprot:
  • P55011()