Western Blot: NKCC1/SLC12A2 Antibody [NB100-75623] - E. coli-derived fusion protein as test antigen. Affinity-purified IgY dilution 1:2000, Goat anti-IgY-HRP dilution 1:1000. Colorimetric method for signal development ...read more
Immunohistochemistry: NKCC1/SLC12A2 Antibody [NB100-75623] - Human colon tissue was incubated with anti-NKCC1 antibody at 5 ug/mL (1:200) overnight at 4C and detected with Alexa Fluor 488 Goat anti-Chicken (1:200 ...read more
Human colon tissue was blocked with 5% BSA and incubated with anti-NKCC1 antibody at 5µg/ml (1:200 dilution [Novus Biologicals, cat# NB100-75623, lot# 118K1873]) overnight at 4°C and detected with Alexa Fluor Goat anti-Chicken 488 (1:200 dilution [Invitrogen, cat# A11039, lot# 745483]). Nuclei were counterstained with DAPI (blue). Cells were imaged using a 20x objective. As negative control, secondary antibody was omitted.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for NKCC1/SLC12A2 Antibody (NB100-75623). (Showing 1 - 1 of 1 FAQ).
Do you have any data with this antibody used on immunohistochemistry?
This particular antibody (NB100-75623) has so far only been tested in western blot. This does not mean that it will not work in IHC, just that we haven't tested it yet. Therefore, there is currently no data using NB100-75623 in IHC. If you would like to go ahead and test this antibody in IHC, note that you would be eligible for our Innovator's Reward program. Alternatively, here are our NKCC1 antibodies that have been validated in IHC.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.