NKCC1/SLC12A2 Antibody

Images

 
Western Blot: NKCC1/SLC12A2 Antibody [NB100-75623] - E. coli-derived fusion protein as test antigen. Affinity-purified IgY dilution 1:2000, Goat anti-IgY-HRP dilution 1:1000. Colorimetric method for signal development ...read more
Immunohistochemistry: NKCC1/SLC12A2 Antibody [NB100-75623] - Human colon tissue was incubated with anti-NKCC1 antibody at 5 ug/mL (1:200) overnight at 4C and detected with Alexa Fluor 488 Goat anti-Chicken (1:200 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NKCC1/SLC12A2 Antibody Summary

Immunogen
The immunogen for this antibody was a sequence from 800a.a. to 1000a.a. from NKCC1(a 1212a.a. long protein)
Localization
Membrane; multi-pass membrane protein.
Specificity
Chicken Polyclonal to NKCC1
Clonality
Polyclonal
Host
Chicken
Gene
SLC12A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry
Application Notes
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution 1:2000. Goat anti-IgY-HRP dilution 1:1000. Colorimetric method for signal development.
Reviewed Applications
Read 1 Review rated 5
using
NB100-75623 in the following applications:

Reactivity Notes

This antibody is reactive against Human, dog, rat, cow, mouse, chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NKCC1/SLC12A2 Antibody

  • Basolateral Na-K-Cl symporter
  • BSC
  • BSC2
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
  • NKCC1
  • NKCC1MGC104233
  • SLC12A2
  • solute carrier family 12 (sodium/potassium/chloride transporters), member 2
  • solute carrier family 12 member 2

Background

NKCC1 (Na

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKCC1/SLC12A2 Antibody (NB100-75623) (0)

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Review for NKCC1/SLC12A2 Antibody (NB100-75623) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-75623:
Filter by Applications
ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry NKCC1/SLC12A2 NB100-75623
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reviewed by:
Edward van Beelen
ICC Human 09/24/2018
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Summary

ApplicationImmunocytochemistry
Sample Testedhuman colon tissue,colon
SpeciesHuman
Lot118K1873

Comments

CommentsHuman colon tissue was blocked with 5% BSA and incubated with anti-NKCC1 antibody at 5µg/ml (1:200 dilution [Novus Biologicals, cat# NB100-75623, lot# 118K1873]) overnight at 4°C and detected with Alexa Fluor Goat anti-Chicken 488 (1:200 dilution [Invitrogen, cat# A11039, lot# 745483]). Nuclei were counterstained with DAPI (blue). Cells were imaged using a 20x objective. As negative control, secondary antibody was omitted.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NKCC1/SLC12A2 Antibody (NB100-75623). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any data with this antibody used on immunohistochemistry?
    • This particular antibody (NB100-75623) has so far only been tested in western blot. This does not mean that it will not work in IHC, just that we haven't tested it yet. Therefore, there is currently no data using NB100-75623 in IHC. If you would like to go ahead and test this antibody in IHC, note that you would be eligible for our Innovator's Reward program. Alternatively, here are our NKCC1 antibodies that have been validated in IHC.

Secondary Antibodies

 

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Bioinformatics Tool for NKCC1/SLC12A2 Antibody (NB100-75623)

Discover related pathways, diseases and genes to NKCC1/SLC12A2 Antibody (NB100-75623). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for NKCC1/SLC12A2 Antibody (NB100-75623)

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Recent Reviews

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1
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Edward van Beelen
09/24/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol SLC12A2
Entrez
Uniprot