NK1R Antibody (694501) [Allophycocyanin]

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human NK1R APC-conjugated Monoclonal Antibody (Catalog # FAB66871A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 694501
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

NK1R Antibody (694501) [Allophycocyanin] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human NK1R
  Accession # P25103
• Specificity: Detects human NK1R in ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: TACR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NK1R Antibody (694501) [Allophycocyanin]

• NK1R
• NK-1R
• NK1Rneurokinin 1 receptor
• NKIR
• SPR
• SPRsubstance-P receptor
• TAC1R
• TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)
• tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
• tachykinin receptor 1NK-1 receptor
• TACR1

Background

NK1R (Neurokinin-1 Receptor), gene name TACR1 (Tachykinin Receptor-1), is an ~80 kDa 7-transmembrane glycoprotein receptor for the proinflammatory tachykinin neuropeptide, Substance P. NK1R is constitutively or inducibly expressed on a wide variety of cells, including monocytes, macrophages, microglia, lymphocytes, neutrophils, mast cells, and neurons. The 407 amino acid (aa) human NK1R contains 89 extracellular aa over multiple segments which collectively share 92% aa identity with corresponding regions of mouse and rat NK1R. An ~50 kDa short isoform that ends at aa 311 lacks almost all of the C-terminal cytoplasmic signaling region. It is the only form expressed in human monocytes and undifferentiated THP-1 cells, and its function appears to differ from the long isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NK1R Antibody (FAB66871A)(1)

Publication using FAB66871A

• A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors Proc. Natl. Acad. Sci. U.S.A., 2017-05-01;0(0):. 2017-05-01 [PMID: 28461492] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TACR1
• Uniprot:
  • P25103()