NIRF Antibody (3A11) - Azide and BSA Free

Western Blot: NIRF Antibody (3A11) [H00115426-M01] - Analysis of UHRF2 over-expressed 293 cell line, cotransfected with UHRF2 Validated Chimera RNAi ( Cat # H00115426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UHRF2 monoclonal antibody (M01), clone 3A11 (Cat # H00115426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunocytochemistry/Immunofluorescence: NIRF Antibody (3A11) [H00115426-M01] - Analysis of monoclonal antibody to UHRF2 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: NIRF Antibody (3A11) [H00115426-M01] - Analysis of UHRF2 expression in transfected 293T cell line by UHRF2 monoclonal antibody (M01), clone 3A11.Lane 1: UHRF2 transfected lysate(56.1 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 3A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NIRF Antibody (3A11) - Azide and BSA Free Summary

• Immunogen: UHRF2 (NP_690856, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGTSTQIEAKPCSNSPPKVKKAPRVGPSNQPSTSARARLIDPGFGIYKVNELVDARDVGLGAWFEAHIHSVTRASDGQSRGKTPLKNGSSCKRTNGNIKH
• Specificity: UHRF2 - ubiquitin-like, containing PHD and RING finger domains, 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UHRF2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It has also been used for RNAi Validation.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NIRF Antibody (3A11) - Azide and BSA Free

• DKFZp434B0920
• DKFZp686G0837
• E3 ubiquitin-protein ligase UHRF2
• EC 6.3.2
• EC 6.3.2.-
• MGC33463
• NIRFUbiquitin-like-containing PHD and RING finger domains protein 2
• Np95/ICBP90-like RING finger protein
• Np95-like RING finger protein
• Nuclear protein 97
• Nuclear zinc finger protein Np97
• RNF107RING finger protein 107
• Ubiquitin-like PHD and RING finger domain-containing protein 2
• ubiquitin-like with PHD and ring finger domains 2
• ubiquitin-like, containing PHD and RING finger domains, 2
• URF2

Background

This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UHRF2
• Entrez:
  • 115426(Human)
• Uniprot:
  • NP_690856(Human)