NIPAL4 Overexpression Lysate

Images

 
Western Blot: NIPAL4 Overexpression Lysate (Adult Normal) [NBP2-08278] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NIPAL4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NIPAL4 Overexpression Lysate Summary

Description
Transient overexpression lysate of NIPA-like domain containing 4 (NIPAL4), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC214908 and based on accession number NM_001099287. The protein contains a C-MYC/DDK Tag.
Gene
NIPAL4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
49.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NIPAL4 Overexpression Lysate

  • NIPA-like domain containing 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34062
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-89409
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-32533
Species: Hu
Applications: WB, IHC, IHC-P
NB100-93466
Species: Hu, Mu, Ca
Applications: IB, ICC/IF, IP, PEP-ELISA
H00126410-B01P
Species: Hu
Applications: WB, ICC/IF
NBP2-46512
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-93476
Species: Hu
Applications: IHC, IHC-P
NBP1-87528
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP2-59320
Species: Hu, Mu, Rt, Ha
Applications: WB, ICC/IF, IHC
NB100-2527
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD
NBP2-16061
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF2940
Species: Hu
Applications: WB, ELISA, Flow, CyTOF-ready
NBP1-89165
Species: Hu
Applications: IHC, IHC-P
NBP1-86461
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-90095
Species: Hu
Applications: IHC, IHC-P
H00728695-B01P
Species: Hu
Applications: WB, ICC/IF, IP
NBP1-88927
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
NBP2-08278
Species: Hu
Applications: WB

Publications for NIPAL4 Lysate (NBP2-08278) (0)

There are no publications for NIPAL4 Lysate (NBP2-08278).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NIPAL4 Lysate (NBP2-08278) (0)

There are no reviews for NIPAL4 Lysate (NBP2-08278). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NIPAL4 Lysate (NBP2-08278) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-08278

Additional NIPAL4 Products

Bioinformatics Tool for NIPAL4 Lysate (NBP2-08278)

Discover related pathways, diseases and genes to NIPAL4 Lysate (NBP2-08278). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NIPAL4 Lysate (NBP2-08278)

Discover more about diseases related to NIPAL4 Lysate (NBP2-08278).
 

Pathways for NIPAL4 Lysate (NBP2-08278)

View related products by pathway.

Blogs on NIPAL4

There are no specific blogs for NIPAL4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

LOX Antibody
NB100-2527

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NIPAL4 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NIPAL4