Niemann-Pick type C2 Antibody

Western Blot: Niemann-Pick type C2 Antibody [NBP1-52028] - Western blot analysis of Niemann-Pick type C2 in Human Testis lysate (35ug protein in RIPA buffer) using NBP1-52028 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Niemann-Pick type C2 Antibody Summary

• Immunogen: Peptide with sequence QAEPVQFKDCGSVD corresponding to internal region according to NP_006423.1.
• Epitope: QAEPVQFKDCGSVD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 20 kDa band and 23 kDa band in human testis lysates. These bands correspond to earlier findings are reported in scientific literature with different antibodies (PMID: 16772431). This protein has a calculated MW of 16.6 kDa band according to NP_006423.1.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Niemann-Pick type C2 Antibody

• EDDM1
• Epididymal Protein 1
• epididymal secretory protein E1
• HE1
• HE1epididymal protein 1
• Human epididymis-specific protein 1
• MGC1333
• Niemann-Pick disease type C2 protein
• Niemann-Pick disease, type C2
• NiemannPick Type C2
• Niemann-Pick Type C2
• NPC2
• NP-C2
• tissue-specific secretory protein

Background

This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Niemann-Pick type C2 Antibody (NBP1-52028)(2)

Publications using NBP1-52028

• Xu Z, Farver W, Kodukula S, Storch J. Regulation of sterol transport between membranes and NPC2. Biochemistry 2008-10-21 [PMID: 18823126]
• Legare C, Thabet M, Gatti JL et al. HE1/NPC2 status in human reproductive tract and ejaculated spermatozoa: consequence of vasectomy. Mol Hum Reprod 2006-07-01 [PMID: 16772431]

FAQs for Niemann-Pick type C2 Antibody (NBP1-52028). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
   • Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01PInnovators Reward Program.

Bioinformatics

• Gene Symbol: NPC2
• Entrez:
  • 10577(Human)
• Uniprot:
  • NP_006423.1(Human)