Niemann-Pick type C2 Antibody [Unconjugated]

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, HepG2 human hepatocellular carcinoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C2/NPC2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Niemann-Pick Type C2/NPC2 was detected in immersion fixed SK-Hep-1 human liver adenocarcinoma cell line using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Niemann-Pick Type C2/NPC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Niemann-Pick type C2 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf21-derived recombinant human Niemann-Pick Type C2/NPC2
  Glu20-Leu151
  Accession # P61916
• Specificity: Detects human Niemann-Pick Type C2/NPC2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPC2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:150 dilution
  • Immunohistochemistry 1:50 dilution
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Niemann-Pick type C2 Antibody [Unconjugated]

• EDDM1
• Epididymal Protein 1
• epididymal secretory protein E1
• HE1
• HE1epididymal protein 1
• Human epididymis-specific protein 1
• MGC1333
• Niemann-Pick disease type C2 protein
• Niemann-Pick disease, type C2
• NiemannPick Type C2
• Niemann-Pick Type C2
• NPC2
• NP-C2
• tissue-specific secretory protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Niemann-Pick type C2 Antibody (AF8644)(2)

Publications using AF8644

• John V. Pluvinage, Jerry Sun, Christel Claes, Ryan A. Flynn, Michael S. Haney, Tal Iram et al. The CD22-IGF2R interaction is a therapeutic target for microglial lysosome dysfunction in Niemann-Pick type C Science Translational Medicine 2021-12-01 [PMID: 34851695]
• Seok-Ho Yu, Tong Wang, Kali Wiggins, Raymond J. Louie, Emilio F. Merino, Cindy Skinner et al. Lysosomal cholesterol accumulation contributes to the movement phenotypes associated with NUS1 haploinsufficiency Genetics in Medicine 2021-07-01 [PMID: 33731878]

FAQs for Niemann-Pick type C2 Antibody (AF8644). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
   • Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01PInnovators Reward Program.

Bioinformatics

• Gene Symbol: NPC2
• Uniprot:
  • P61916()