Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody [NBP1-78791] - (1ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody Summary

• Immunogen: Peptide with sequence C-HVDRYFTQKEET corresponding to internal region according to NP_000736.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHRNA5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in KELLY lysate (calculated MW of 53.1 kDa band according to NP_000736.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody

• Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5
• cholinergic receptor, nicotinic, alpha 5
• cholinergic receptor, nicotinic, alpha polypeptide 5
• CHRNA5
• LNCR2
• NACHRA5
• neuronal acetylcholine receptor subunit alpha-5
• neuronal nicotinic acetylcholine receptor, alpha5 subunit
• Nicotinic Acetylcholine R alpha 5
• Nicotinic Acetylcholine Ra5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (NBP1-78791)(1)

Publication using NBP1-78791

• Kuryatov A, Berrettini W, Lindstrom J. Acetylcholine receptor (AChR) a5 subunit variant associated with risk for nicotine dependence and lung cancer reduces (a4beta2)2) 2 a5 AChR function. Mol Pharmacol 2011-01-01 [PMID: 20881005]

FAQs for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (NBP1-78791). (Showing 1 - 1 of 1 FAQ).

1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
   • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Bioinformatics

• Gene Symbol: CHRNA5
• Entrez:
  • 1138(Human)
• Uniprot:
  • NP_000736.2(Human)