Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

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Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143] - Analysis of Nicotinic Acetylcholine Receptor alpha 4 in Rat Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.3ug/ml. ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, PEP-ELISA, IP (-)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody Summary

Immunogen
Peptide with sequence C-HVETRAHAEERLLKK corresponding to internal region according to NP_000735.1.
Epitope
internal region
Specificity
This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHRNA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
IP: Not suitable. WB: Approx. 70 kDa band observed in rat brain lysates (calculated MW of 70.0 kDa band according to human NP_000735.1and of 70.2 kDa band according to rat NP_077330.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide. An anonymous customer has reproted ~75 kDa band in both human and rat tissues as the glycoprotein.
Positive Control
Nicotinic Acetylcholine R alpha 4/CHRNA4 Lysate (NBL1-09181)
Publications
Read Publication using NBP1-00143.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

  • nAchRa4
  • BFNC
  • cholinergic receptor, nicotinic, alpha 4
  • cholinergic receptor, nicotinic, alpha polypeptide 4
  • CHRNA4
  • EBN
  • EBN1
  • ENFL1
  • FLJ95812
  • nAChR alpha 4
  • NACHR
  • NACHRA4
  • NACRA4
  • neuronal acetylcholine receptor subunit alpha-4
  • neuronal nicotinic acetylcholine receptor alpha-4 subunit
  • Nicotinic Acetylcholine R alpha 4
  • Nicotinic Acetylcholine Ra4

Background

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (NBP1-00143)(1)

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FAQs for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (NBP1-00143). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
    • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHRNA4
Entrez
Uniprot