NGFRAP1/BEX3/NADE Antibody

Product Discontinued

NGFRAP1/BEX3/NADE Antibody Summary

• Immunogen: Peptide with sequence CLRILMGELSNHHDH corresponding to C-Terminus according to NP_996800.1, NP_996798.1, NP_055195.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognise isoform a (NP_996800.1) and isoform b (NP_996798.1 and NP_055195.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BEX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments in human liver, ovary, prostate and testis lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for NGFRAP1/BEX3/NADE Antibody

• BEX3
• BEX3p75NTR-associated cell death executor
• Brain-expressed X-linked protein 3
• DXS6984EX-linked 3
• HGR74
• NADE
• nerve growth factor receptor (TNFRSF16) associated protein 1
• Nerve growth factor receptor-associated protein 1
• NGFRAP1
• Ovarian granulosa cell 13.0 kDa protein HGR74
• ovarian granulosa cell protein (13kD)
• protein BEX3

Background

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Coexpression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NGFRAP1/BEX3/NADE Antibody (NBP1-20952)(1)

Publication using NBP1-20952

• Passino MA, Adams RA, Sikorski SL, Akassoglou K. Regulation of hepatic stellate cell differentiation by the neurotrophin receptor p75NTR. Science 2007-03-30 [PMID: 17395831]

Bioinformatics

• Gene Symbol: BEX3
• Entrez:
  • 27018(Human)
  • 12070(Mouse)
  • 117089(Rat)
• Uniprot:
  • NP_996800.1(Human)