NGFRAP1/BEX3/NADE Antibody

Western Blot: NGFRAP1/BEX3/NADE Antibody [H00027018-B01P] - Analysis of NGFRAP1 expression in transfected 293T cell line by NGFRAP1 polyclonal antibody. Lane 1: NGFRAP1 transfected lysate(11.11 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NGFRAP1/BEX3/NADE Antibody [H00027018-B01P] - Analysis of purified antibody to NGFRAP1 on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

NGFRAP1/BEX3/NADE Antibody Summary

• Immunogen: NGFRAP1 (NP_996800.1, 1 a.a. - 101 a.a.) full-length human protein. MEQPMQNGEEDRPLGGGEGHQPAGNRRGQARRLAPNFRWAIPNRQINDGMGGDGDDMEIFMEEMREIRRKLRELQLRNCLRILMGELSNHHDHHDEFCLMP
• Specificity: NGFRAP1 - nerve growth factor receptor (TNFRSF16) associated protein 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: BEX3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NGFRAP1/BEX3/NADE Antibody

• BEX3
• BEX3p75NTR-associated cell death executor
• Brain-expressed X-linked protein 3
• DXS6984EX-linked 3
• HGR74
• NADE
• nerve growth factor receptor (TNFRSF16) associated protein 1
• Nerve growth factor receptor-associated protein 1
• NGFRAP1
• Ovarian granulosa cell 13.0 kDa protein HGR74
• ovarian granulosa cell protein (13kD)
• protein BEX3

Background

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Coexpression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BEX3
• Entrez:
  • 27018(Human)
• Uniprot:
  • NP_996800.1(Human)