NGFI-B alpha/Nur77/NR4A1 Antibody

Product Discontinued

NGFI-B alpha/Nur77/NR4A1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 320-365 of Human Nur77/NR4A1.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of NUR77/NR4A1 (G347) pAb in extracts from Jurkat cells.
  Immunohistochemistry (IHC) analyzes of NUR77/NR4A1 (G347) pAb in paraffin-embedded human colon carcinoma tissue.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody

• Early response protein NAK1
• GFRP1ST-59
• growth factor-inducible nuclear protein N10
• HMR
• HMRNP10
• hormone receptor
• MGC9485
• N10
• NAK1
• NAK-1
• NGFIB alpha
• NGFI-B alpha
• NGFIB
• NR4A1
• Nuclear hormone receptor NUR/77
• nuclear receptor subfamily 4 group A member 1
• nuclear receptor subfamily 4, group A, member 1
• Nur77
• Orphan nuclear receptor HMR
• Orphan nuclear receptor TR3
• steroid receptor TR3
• Testicular receptor 3
• TR3 orphan receptor
• TR3

Background

Nurr1 (Nur-related factor 1) and NUR77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and NUR77are growth factor inducible immediate early response genes. Induction of both Nurr1 and NUR77 is seen after membrane depolarization while only NUR77 induction is seen with NGF stimulation. JunD acts as a mediator for NUR77. An increase in NUR77expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, NUR77may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of NUR77to bind DNA. A dominant negative form of NUR77can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of NUR77for TCR-mediated apoptosis is still under debate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR4A1
• Entrez:
  • 3164(Human)
  • 15370(Mouse)
  • 79240(Rat)
• Uniprot:
  • P22736(Human)