NFkB p105/p50 Antibody (A2-7)

Western Blot: NFkB p105/p50 Antibody (A2-7) [NBP2-66976] - Analysis of NFkB p105 / p50 on MCF-7 cell lysate.

Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody (A2-7) [NBP2-66976] - Staining NFkB p105 / p50 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (A2-7) [NBP2-66976] - Analysis of human breast carcinoma tissue using anti- NFkB p105 / p50 antibody. Counter stained with hematoxylin.

Product Discontinued

NFkB p105/p50 Antibody (A2-7) Summary

• Immunogen: Synthetic peptide within Human NFkB p105/p50 aa 317-366 / 3968. (SwissProt: P19838 Human)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFKB1
• Purity: Protein L purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:1000-1:2000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein L purified

Alternate Names for NFkB p105/p50 Antibody (A2-7)

• DKFZp686C01211
• DNA binding factor KBF1
• DNA-binding factor KBF1
• EBP-1
• KBF1
• NF-kappaB
• NF-kappa-B
• NF-kappabeta
• NFKB-p105
• NFKB-p50
• nuclear factor kappa-B DNA binding subunit
• nuclear factor NF-kappa-B p105 subunit
• nuclear factor NF-kappa-B p50 subunit
• nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
• p105
• p50

Background

NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFkB p105/p50 Antibody (NBP2-66976)(1)

Publication using NBP2-66976

• Aladily TN, Abushunar T, Alhesa A et al. Immunohistochemical Expression Patterns of CD45RO, p105/p50, JAK3, TOX, and IL-17 in Early-Stage Mycosis Fungoides Diagnostics (Basel, Switzerland) 2022-01-17 [PMID: 35054387] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: NFKB1