NFkB p105/p50 Antibody (5D10)

Western Blot: NFkB p105/p50 Antibody (5D10) [NBP2-22202] - Western blot analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (5D10) [NBP2-22202] - Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and bladder cancer tissues (right) using NFkB p105/p50 mouse mAb with DAB staining.

Flow Cytometry: NFkB p105/p50 Antibody (5D10) [NBP2-22202] - Flow cytometric analysis of MCF-7 cells using NFkB p105/p50 mouse mAb (green) and negative control (purple).

ELISA: NFkB p105/p50 Antibody (5D10) [NBP2-22202] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Discontinued

NFkB p105/p50 Antibody (5D10) Summary

• Immunogen: Purified recombinant fragment of human NFKB1 expressed in E. Coli.
• Localization: Cytoplasm
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFKB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:2000
• Theoretical MW: 50/105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for NFkB p105/p50 Antibody (5D10)

• DKFZp686C01211
• DNA binding factor KBF1
• DNA-binding factor KBF1
• EBP-1
• KBF1
• NF-kappaB
• NF-kappa-B
• NF-kappabeta
• NFKB-p105
• NFKB-p50
• nuclear factor kappa-B DNA binding subunit
• nuclear factor NF-kappa-B p105 subunit
• nuclear factor NF-kappa-B p50 subunit
• nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
• p105
• p50

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFkB p105/p50 Antibody (NBP2-22202)(1)

Publication using NBP2-22202

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: NFKB1