NFIX Antibody

Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15038] - Mouse hippocampus. NFIX stained by NFIX antibody [N2C2], Internal diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15038] - Rat hippocampus. NFIX stained by NFIX antibody [N2C2], Internal diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: NFIX Antibody [NBP2-15038] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIX antibody [N2C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

NFIX antibody [N2C2], Internal detects NFIX protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NFIX stained by NFIX antibody [N2C2], Internal (NBP2-15038) diluted at 1:500.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NFIX Antibody Summary

• Description: Novus Biologicals Rabbit NFIX Antibody (NBP2-15038) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-NFIX Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFIX
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NFIX Antibody

• CTF
• NF1ACCAAT-box-binding transcription factor
• NF1-X
• NF-I/X
• NFI-X
• nuclear factor 1 X-type
• Nuclear factor 1/X
• nuclear factor I/X (CCAAT-binding transcription factor)
• Nuclear factor I/X
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NFIX Antibody (NBP2-15038)(4)

Publications using NBP2-15038

• Martins SG, Ribeiro V, Melo C et al. Laminin-alpha 2 chain deficiency in skeletal muscle causes dysregulation of multiple cellular mechanisms Life Science Alliance 2024-10-08 [PMID: 39379105]
• Giuseppe Angelini, Emanuele Capra, Francesca Rossi, Giada Mura, Marielle Saclier, Valentina Taglietti, Gabriele Rovetta, Raffaele Epis, Giorgia Careccia, Chiara Bonfanti, Graziella Messina MEK-inhibitors decrease Nfix in muscular dystrophy but induce unexpected calcifications, partially rescued with Cyanidin diet iScience 2023-12-10 [PMID: 38205246]
• Saclier M, Angelini G, Bonfanti C et al. Selective ablation of Nfix in macrophages attenuates muscular dystrophy by inhibiting fibro-adipogenic progenitor-dependent fibrosis The Journal of pathology 2022-03-17 [PMID: 35297529] (Immunohistochemistry, Mouse)
• Piper M, Gronostajski R, Messina G. Nuclear Factor One X in Development and Disease. Trends Cell Biol. 2018-10-01 [PMID: 30287093] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: NFIX
• Uniprot:
  • Q14938(Human)