NFIX Antibody (3D2) - Azide and BSA Free

Western Blot: NFIX Antibody (3D2) [H00004784-M08] - NFIX monoclonal antibody (M08), clone 3D2. Western Blot analysis of NFIX expression in A-431.

Western Blot: NFIX Antibody (3D2) [H00004784-M08] - Analysis of NFIX expression in Jurkat (Cat # L017V1).

ELISA: NFIX Antibody (3D2) [H00004784-M08] - Detection limit for recombinant GST tagged NFIX is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NFIX Antibody (3D2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NFIX (NP_002492.2, 291 a.a. ~ 390 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSSQGSSPRMAFTHHPLPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIR
• Specificity: Reacts with nuclear factor I/X (CCAAT-binding transcription factor).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFIX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NFIX Antibody (3D2) - Azide and BSA Free

• CTF
• NF1ACCAAT-box-binding transcription factor
• NF1-X
• NF-I/X
• NFI-X
• nuclear factor 1 X-type
• Nuclear factor 1/X
• nuclear factor I/X (CCAAT-binding transcription factor)
• Nuclear factor I/X
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFIX
• Entrez:
  • 4784(Human)
• Uniprot:
  • NP_002492(Human)