NFIB Antibody (2D6) - Azide and BSA Free

Western Blot: NFIB Antibody (2D6) [H00004781-M08] - Analysis of NFIB expression in transfected 293T cell line by NFIB monoclonal antibody (M08), clone 2D6. Lane 1: NFIB transfected lysatE (47.4 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NFIB Antibody (2D6) [H00004781-M08] - Analysis of monoclonal antibody to NFIB on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: NFIB Antibody (2D6) [H00004781-M08] - Analysis of monoclonal antibody to NFIB on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NFIB Antibody (2D6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NFIB (AAH01283, 1 a.a. ~ 420 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHNDPAKNPPGYLEDSFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQSWYLG
• Specificity: NFIB - nuclear factor I/B (2D6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFIB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NFIB Antibody (2D6) - Azide and BSA Free

• CCAAT-box-binding transcription factor
• CTF
• HMGIC/NFIB
• NF-I/B
• NFI-B
• NFIB2
• NFIB3
• NFI-RED
• nuclear factor 1 B-type
• Nuclear factor 1/B
• nuclear factor I/BNF1-B
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFIB Antibody (H00004781-M08)(1)

Publication using H00004781-M08

• Moon HG, Hwang KT, Kim JA et al. NFIB is a potential target for estrogen receptor-negative breast cancers. Molecular Oncology 2011-01-01 [PMID: 21925980]

Bioinformatics

• Gene Symbol: NFIB
• Entrez:
  • 4781(Human)
• Uniprot:
  • AAH01283(Human)