NF2/Merlin Antibody [Alexa Fluor® 594]

• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

NF2/Merlin Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human NF2
  Met363-Lys578
  Accession # P35240
• Specificity: Detects human NF2 in Western blots and direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

NF2 (Neurofibromin-2; also Merlin) is a 70‑75 kDa tumor suppressor protein belonging to the ERM (ezrin, radixin, moesin) family of proteins. It participates in contact‑mediated cell adhesion, thereby blocking cell proliferation and migration. Human NF2 is 595 amino acids (aa) in length. It contains three general regions. There is an N‑terminal FERM domain (aa 1‑302), a alpha -helical rod central region (aa 303‑478), and a unique carboxy-terminal domain (aa 479‑595). Confluence requires an absence of phosphorylation at Ser516 and Ser10. There are multiple potential splice variants. Four short forms less that 260 aa in length lack the aa range used for antigen immunization. There are at least eight additional isoforms that basically show an alternate start site coupled to an 11 aa substitution for aa 579‑595. Lack of NF2 is associated with neurofibromatosis type II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.