Human Neurotrypsin ELISA Kit (Colorimetric)

Standard Curve Reference.

Samples were spiked with high concentrations of Human Neurotrypsin, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Hu
• Applications: ELISA
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Human Neurotrypsin ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 3 h 30 min
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PRSS12

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Neurotrypsin ELISA Kit (Colorimetric)

• brain-specific serine protease 3
• BSSP3
• BSSP-3
• EC 3.4.21
• Leydin
• MGC12722
• Motopsin
• MRT1EC 3.4.21.-
• neurotrypsin
• protease, serine, 12 (neurotrypsin, motopsin)
• Serine protease 12

Background

Neurotrypsin is a central nervous system-expressed serine protease whose truncation or absence causes nonsyndromic mental retardation. It is most prominently expressed in structures that are involved in the processing and storage of learned behavior and memory, such as the cerebral cortex, the hippocampus, and amygdala. Evidence suggests that neurotrypsin has multiple functions, including axonal outgrowth, maintaining neuronal plasticity, and arranging the perineuronal environment, partly in coordination with other proteases including tissue plasminogen activator. There are three sequences published for neurotrypsin to date; 875, 834 and 505 amino acids in length. The predicted masses are 97, 92.5 and 55.7 kDa respectively, with predicted pI of 9.14, 8.88 and 6.57. The three forms all start at the same place. The 505 residue form terminates in the last SRCR domain, before the catalytic domain, and the 834 amino acid form has a 40 amino acid deletion that starts just after the PC site, and includes the catalytic histidine, thus both species would be proteolytically inactive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Neurotrypsin ELISA Kit (NBP3-49892). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: PRSS12