VCX3A Antibody (6A3) - Azide and BSA Free

Western Blot: VCX3A Antibody (6A3) [H00051481-M01] - Analysis of VCX3A expression in transfected 293T cell line by VCX3A monoclonal antibody (M01), clone 6A3.Lane 1: VCX3A transfected lysate(17.8 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: VCX3A Antibody (6A3) [H00051481-M01] - Detection limit for recombinant GST tagged VCX3A is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 6A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VCX3A Antibody (6A3) - Azide and BSA Free Summary

• Immunogen: VCX3A (NP_057463, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPKPRASGPPAKATEAGKRKSSSQPSPSDPKKKTTKVAKKGKAVRRGRRGKKGAATKMAAVTAPEAESGPAAPGPSDQPSQELPQHELPPEEPVSEGTQ
• Specificity: VCX3A - variable charge, X-linked 3A
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCX3A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VCX3A Antibody (6A3) - Azide and BSA Free

• Variable charge protein on X with eight repeats
• variable charge X-linked protein 3
• variable charge, X-linked 3A
• Variably charged protein X-A
• variably charged X-A
• VCX3
• VCX-8R
• VCX8RMGC125730
• VCX-8rvariable charge, X-linked 3
• VCX-AMGC118976
• VCXAMGC125796

Background

This gene belongs to the VCX/Y gene family, which has multiple members on both X and Y chromosomes, and all are expressed exclusively in male germ cells. The X-linked members are clustered on chromosome Xp22 and Y-linked members are two identical copies of the gene within a palindromic region on Yq11. The family members share a high degree of sequence identity, with the exception that a 30-bp unit is tandemly repeated in X-linked members but occurs only once in Y-linked members. The VCX gene cluster is polymorphic in terms of copy number; different individuals may have a different number of VCX genes. VCX/Y genes encode small and highly charged proteins of unknown function. The presence of a putative bipartite nuclear localization signal suggests that VCX/Y members are nuclear proteins. This gene contains 8 repeats of the 30-bp unit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCX3A Antibody (H00051481-M01)(1)

Publication using H00051481-M01

• Deng H, Zeng J, Zhang T et al. Histone H3.3K27M Mobilizes Multiple Cancer/Testis (CT) Antigens in Pediatric Glioma. Mol Cancer Res 2018-04-01 [PMID: 29453317]

Bioinformatics

• Gene Symbol: VCX3A
• Entrez:
  • 51481(Human)
• OMIM:
  • 300533(Human)
• Uniprot:
  • NP_057463(Human)